Fig. 6: hkb functions through eve to regulate DIP-α-EGFP expression and m4-Is innervation in embryos, 1st, and 2nd instar stages.
From: hkb is required for DIP-α expression and target recognition in the Drosophila neuromuscular circuit
Representative images of dorsal Is MN cell bodies (arrows in a) and ventral Is MN cell bodies (arrows in a’) labeled with GFP (green), Eve (red), and FasII (magenta) in control embryos. DIP-α-EGFP is expressed in both dorsal and ventral Is MNs. Representative images of dorsal Is MN cell bodies (dashed circles in b) and ventral Is MN cell bodies (arrows in b’) in eveΔRN2 mutant embryos. Eve and DIP-α-EGFP are missing in dorsal Is MNs, whereas DIP-α-EGFP expression in ventral Is MNs is not affected. Representative images of dorsal Is MN cell bodies (arrows in c) and ventral Is MN cell bodies (arrows in c’) labeled with GFP (green), Eve (red), and HRP (magenta) in control 1st instar larvae. DIP-α-EGFP is expressed in both dorsal and ventral Is MNs. Representative images of dorsal Is MN cell bodies (dashed circles in d) and ventral Is MN cell bodies (arrows in d’) in eveΔRN2 mutant 1st instar larvae. Eve and DIP-α-EGFP are missing in dorsal Is MNs, whereas DIP-α-EGFP expression in ventral Is MNs is not affected. e Quantification of DIP-α-EGFP expression in dorsal Is MN cell bodies in control and eveΔRN2 mutant larvae. N (ROI with two cell bodies) = 25 and 25. p value is indicated. Error bar indicates standard error of the mean (SEM). Representative images of NMJs formed by Ib MN, dorsal Is MN, and ventral Is MN (arrows) labeled with GFP (green), DLG (red), HRP (magenta), and Phalloidin (Blue), in 1st instar DIP-α-EGFP expressing larvae in (f) control and (g) eveΔRN2 mutant background. h Quantification of m4-Is innervation frequency in 1st instar control and eveΔRN2 mutant larvae. N (NMJs) = 27 and 25. p value is indicated. Representative images of NMJs formed by the Ib MN, dorsal Is MN, and ventral Is MN (arrows) labeled with GFP (green), DLG (red), HRP (magenta), and Phalloidin (Blue), in 2nd instar DIP-α-EGFP expressing larvae in (i) control and (j) eveΔRN2 mutant background. k Quantification of m4-Is innervation frequency in 2nd instar control and eveΔRN2 mutant larvae. N (NMJs) = 53 and 44. p value is indicated.
