Fig. 1: Circ-007530 significantly downregulated in GC.

a Circ-0075305 host gene chromosome location and Sanger sequencing verified the Circ-0075305 back splicing structure. b Detection of Circ-0075305 expression in gastric cancer cell lines using qRT-PCR. c Stability of CircRNA in HGC-27 cells was verified using an actinomycin D assay. d The characteristics of CircRNAs in HGC-27 cells that were not easily digested were verified using RNase R exonuclease digestion experiments. e The cyclic properties of CircRNAs in HGC-27 cells were verified by DNA gel electrophoresis. f The relative expression of Circ-0075305 in 200 pairs of human gastric cancer tissues using RT-qPCR. g Circ-0075305 expression and one-to-one pairing were detected using qRT-PCR. h Analysis of the impact of Circ-0075305 expression on the OS rate of patients with GC. i Analysis of the impact of Circ-0075305 expression on the OS rate in postoperative chemotherapy patients with GC. j The locations of CircRNAs were verified by performing nucleoplasmic separation experiments in HGC-27. Data are represented as the mean ± SD and analyzed by Student’s t-test or one-way analysis of variance (ANOVA). NS, no significance, *P < 0.05, **P < 0.01, ***P < 0.001 for groups connected by horizontal lines. P-values < 0.05 were considered statistically significant. c, d, j: n = 3 per group; b: n = 5 per group.