Fig. 2: In vitro and in vivo investigation of the impact of Circ-0075305 up-regulation on chemotherapy sensitivity reduction in GC cells. | Communications Biology

Fig. 2: In vitro and in vivo investigation of the impact of Circ-0075305 up-regulation on chemotherapy sensitivity reduction in GC cells.

From: Circ-0075305 hinders gastric cancer stem cells by indirectly disrupting TCF4–β-catenin complex and downregulation of SOX9

Fig. 2: In vitro and in vivo investigation of the impact of Circ-0075305 up-regulation on chemotherapy sensitivity reduction in GC cells.

a The transfection efficiency of overexpressed and knocked down Circ-0075305 in gastric cancer cell lines was assessed using qRT-PCR. b, c The CCK8 assay was used to monitor the growth of BGC-823 and HGC-27 cells. d, e The CCK8 assay was used to monitor alterations in the sublethal concentration (IC50) of the chemotherapy drug (OXA) in the Circ-0075305 GC cell line. f Schematic representation of nude mice treated with DMSO/OXA. g Subcutaneous tumor formation in mice inoculated with Circ-0075305 GC cells after stable transfer of OXA/DMSO treatment. h The changes of tumor weight were recorded. i The changes of tumor volume were recorded. j The distribution of HE and Ki-67 expression in tumors was detected by using immunohistochemistry. k Statistical analysis of Ki-67 positive cells in tumor tissues. Data are represented as the mean ± SD and analyzed by Student’s t-test or one-way analysis of variance (ANOVA). *P < 0.05, **P < 0.01, ***P < 0.001 for groups connected by horizontal lines. P-values < 0.05 were considered statistically significant. a–d, e, k: n = 3 per group; h, i: n = 5 per group.

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