Fig. 3: Up-regulation of Circ-0075305 can reduce stem-like properties of GC in vitro and in vivo. | Communications Biology

Fig. 3: Up-regulation of Circ-0075305 can reduce stem-like properties of GC in vitro and in vivo.

From: Circ-0075305 hinders gastric cancer stem cells by indirectly disrupting TCF4–β-catenin complex and downregulation of SOX9

Fig. 3: Up-regulation of Circ-0075305 can reduce stem-like properties of GC in vitro and in vivo.

a Tumor formation frequencies for different numbers of the BGC-823 cells with or without Circ-0075305 knockdown. b The dilution of a was statistically analyzed for tumor formation. c The changes of tumor weight were recorded. d The changes of tumor volume were recorded. e Flow cytometry reveals the percentage of CD44+ cells within the HGC-27 and BGC-823 cell populations, following transfection with either the control vector or Circ-0075305 for overexpression or suppression. f Quantitative assessment of the flow cytometry results from the experiment conducted in e. g Immunofluorescence images of CD44 and NANOG were obtained from gastric cancer organoids transfected with a vector, Circ-0075305 overexpression or Circ-0075305 knockdown. h The proportions of CD44 and NANOG positive GC organoids in g were statistically analyzed. i Western blot was used to analyze the effect of Circ-0075305 on gastric cancer stem cell-like characteristics. j Statistical interpretation of the results showcased in i. Data are represented as the mean ± SD and analyzed by Student’s t-test. **P < 0.01, ***P < 0.001 for groups connected by horizontal lines. P-values < 0.05 were considered statistically significant. f, h, j: n = 3 per group; c, d: n = 5 per group.

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