Fig. 6: Circ-0075305/miR-708-5p/RPRD1A co-regulated stem cell-like properties of GC.

a Immunofluorescence images of CD44 and NANOG were obtained from gastric cancer organoids transfected with a vector, Circ-0075305 overexpression, miR-708-5p mimic expression, or both Circ-0075305 overexpression and miR-708-5p mimic expression. b The proportions of CD44 and NANOG positive GC organoids in a were statistically analyzed. c Immunofluorescence images of CD44 and NANOG were obtained from gastric cancer organoids transfected with a vector, Circ-0075305 overexpression, RPRD1A knockdown, or both. d The proportions of CD44 and NANOG positive GC organoids in Fig. c were statistically analyzed. e Western blot analysis was employed to detect alterations in stem cell-like property marker expression in GC cells transfected with Circ-0075305 or/and miR-708-5p mimic. f Quantitative interpretation of the Western blot results from e. g Western blot analysis of stem cell-like property marker expression in GC cells transfected with Circ-0075305 or/and sh-RPRD1A. h Quantitative interpretation of the Western blot results from g. i Proportion of CD44+ cells within HGC-27 and BGC-823 cell populations, transfected with Circ-0075305 or/and miR-708-5p mimic, identified via flow cytometry. j Statistical analysis of the results in i. k Immunohistochemical detection of CD44 and SOX9 expression in tumors samples. l Quantitative assessment and correlation analysis of the indicators observed in k. m Immunohistochemical detection of the distribution of CD44 and SOX9 expression in tumors samples. n Statistical analysis of the relationships between the expression of the indicators in m. Data are represented as the mean ± SD and analyzed by one-way analysis of variance (ANOVA). *P < 0.05, **P < 0.01, ***P < 0.001 for groups connected by horizontal lines. P-values < 0.05 were considered statistically significant. b, d, f, h, j, i, n: n = 3 per group; b: n = 5 per group.