Fig. 2: Cryo-EM analysis of AFP.

a A raw micrograph of the frozen-hydrated AFP taken from the 300 kV Titan Krios microscope. b Typical 2D class averages of AFP. c Selected densities from the AFP map with the corresponding atomic models docked. d The final 3D reconstruction map of AFP at 3.31 Å resolution. e The overall cryo-EM structure of AFP.