Fig. 3: N-glycosylation of AFP.

a Additional electron density map linking to the Asn251 of AFP. The density map is color-coded according to AFP subdomain, and the map of the extra electron density map is marked in red. AFP sequences conform to the N-linked glycosylation sequence pattern. b SDS-PAGE analysis of AFP and mutant AFP (N251S). c Oligosaccharide structure of AFP. The oligosaccharide is called beta - D - mannopyranose - (1-4) - 2 - acetamido - 2 - deoxy - beta - D - glucopyranose - (1-4) - 2 - acetamido - 2 - deoxy - beta - D - glucopyranose.