Table 1 Data processing details, refinement, and validation statistics
AFP (EMD-37997, PDB: 8X1N) | |
|---|---|
Data collection and processing | |
Magnification | 165,000× |
Voltage (kV) | 300 |
Electron exposure (e-/Å2) | 50 |
Defocus range (µm) | 1.0–1.6 |
Pixel size (Å) | 0.526 |
Symmetry imposed | C1 |
Micrographs | 10,849 |
Initial particle images (no.) | 739,577 |
Final particle images (no.) | 144,221 |
Resolution offinal map [Å] | 3.31 |
FSC threshold | 0.143 |
B-factor applied [Å2] | −149.8 |
Refinement | |
Model composition | |
Non-hydrogen atoms | 4763 |
Protein residues | 591 |
R.m.s deviations | |
Bond lengths (Å) | 0.003 |
Bond angles (°) | 0.549 |
Validation | |
MolProbity score | 1.15 |
Clashscore | 2.85 |
Poor rotamers (%) | 0.00 |
Ramachandran plot | |
Favored (%) | 97.62 |
Allowed (%) | 2.38 |
Disallowed (%) | 0.00 |
