Fig. 2: VisuAlign and QCAlign were used to refine and verify the regional atlas-registration achieved in the QUINT workflow.
From: Detecting the effect of genetic diversity on brain composition in an Alzheimer’s disease mouse model
a VisuAlign GUI displaying one thionine section with nonlinear refinements applied to achieve an improved match of the atlas delineations over the section. CCFv3 regional borders are overlaid on the section with the position of the borders manipulated using anchor points. The lines indicate the start position of the points prior to nonlinear refinement, with the black markers denoting their final position after nonlinear refinement. (i). Inset displaying the atlas-registration achieved by linear registration using QuickNII. The dentate gyrus cell layers are incorrectly positioned over the section. (ii). Inset displaying the atlas-registration achieved using QuickNII and VisuAlign. The positioning of the dentate gyrus cell layers has been adjusted to match the cell layers in the section. b QCAlign GUI displaying one thionine section with a grid of systematic random sampling points overlaid. Grid points are marked up as registered accurately (+) or inaccurately (−) based on the region name, which is displayed in the GUI by hovering over a point (region name shown for point indicated with the arrow). (iii). Inset displaying the quality of the atlas-registration achieved by linear registration with QuickNII only (87% accurate for the inset) (iii). Inset displaying the quality of the atlas-registration achieved by registration with QuickNII and VisuAlign (with nonlinear refinement) (100% accurate for inset iv.).
