Fig. 2: Ptr_b is a race-specific resistance allele.

a Multiple sequence alignment of the polymorphic 79-83 C-terminal amino acids of Ptr variants with polymorphic residues relative to the Katy sequence (Ptr_a) shown as single letter AA code with deletions shown as dashes and identical residues shown as dots. The Mo15-23 and Mo15-24 (group two) resistance-associated rice accessions are shown in green, the IK81-25, M64-1-3-9-1, and Mo15-125 (group one) resistance-associated rice accessions are shown in yellow, and the IRIS_313-12190 accession, which carried resistance associations to both group one and group two isolates at chromosome 12 is shown in gray. A 4 AA deletion that was previously reported to be diagnostic for Ptr_a is indicated by a blue box. The genotypes presented are classified into five variant subgroups (Ptr_a – Ptr_e; indicated in letters a-e to the left of genotype names) based on the allelic diversity observed in 31 genetically diverse genotypes. b Four-week-old Ptr_b containing transgenic lines (3 and 16), their non-transgenic siblings (3_sib and 16_sib), and the susceptible parental line CO39 were spray-inoculated with M. oryzae isolates indicated. Images of representative infected leaves were taken 7 days post-inoculation. Green boxes indicate transgenic plants containing Ptr_b. Scale bar = 2 cm. c Infection scoring data from five plants each of 4-week-old Ptr_b containing transgenic lines (3 and 16) and their non-transgenic siblings (3_sib and 16_sib). Scoring data at 7 days post inoculation is represented by box plots where diamonds indicate the average infection score for each genotype. Significant differences between sibling lines infected with each isolate, as determined by a two-sample t-test, are indicated (*** = P < 0.001), n = 5 plants.