Fig. 4: PA induces lipid accumulation and NP cell senescence in an ER-stress-dependent manner. | Communications Biology

Fig. 4: PA induces lipid accumulation and NP cell senescence in an ER-stress-dependent manner.

From: Palmitic acid induces lipid droplet accumulation and senescence in nucleus pulposus cells via ER-stress pathway

Fig. 4

NP cells were incubated with or without the ER-stress-specific inhibitor 4-PBA and 200 μM PA for 24 h. a–c GRP78 and CHOP protein levels were assessed by WB, PCR, and IF respectively. n = 3. **P < 0.01; ***P < 0.001. Scale bar: 10 μm. d BODIPY staining for lipid droplets. Scale bar: 10 μm. e Ultrastructural view of ER and lipid droplets using TEM. Scale bar: 0.5 μm. f, g Oil red staining for lipid droplets. Scale bar: 100 μm. The positive oil red staining cells are shown in the statistical chart; n = 3. **P < 0.01; ***P < 0.001. h–k P53, P21, P16, and RB levels were assessed by WB, PCR, and IF respectively. n = 3. **P < 0.01; ***P < 0.001. Scale bar: 50 μm. l Representative SA-β-gal staining of NP cells. Scale bar: 100 μm. m EdU staining detected the proliferation of NP cells. Scale bar: 100 μm. n, o The positive EdU- and SA-β-gal cells are shown in the statistical chart. n = 3. **P < 0.01; ***P < 0.001.

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