Fig. 2: Modified Vaccinia virus Ankara (MVA) infection induced progressive changes in Vero host chromatin 3D architecture.

a Hi-C contact matrices of chromosome 27 in mock-infected control (lower diagonal region) versus MVA-infected (upper diagonal region) cells at 12, 18, and 24 hours post infection (hpi). Green boxes indicate regions with pronounced contact differences between control and MVA-infected cells, where the bin size is 100 kb. b Differential (infected/control) contact matrices of chromosome 27 across all three time points are shown at 100 kb resolution. Blue indicates control-biased contacts, where more contacts were observed in control cells, and red indicates infection-biased contacts, where more contacts were observed in MVA-infected cells. c) Pearson correlation matrix of chromosome 27 in mock-infected control (lower diagonal region) versus MVA-infected (upper diagonal region) cells, where bin size is 100 kb. Red boxes highlight the regions with altered compartmentalization. d Scatterplots demonstrating replicate averaged compartment scores (eigen value) of control vs infected cells in 100 kb bins across the genome at all three time points. Off-diagonal points represents the compartment exchange events, where orange indicates A to B compartment exchange and purple indicates B to A compartment exchange. Counts of compartment exchange are annotated along the off diagonal. The Jaccard index is annotated in the bottom left and measures the overlap between compartment assignments between mock-infected control and MVA-infected scores.