Fig. 1: Substrate specificity analysis of NSD2.
From: Discovery of NSD2 non-histone substrates and design of a super-substrate
a Autoradiographic image (3 days of exposure) of a peptide SPOT array methylated by NSD2 in the presence of radioactively labeled AdoMet. The horizontal axis represents the H3K36 (29-43) template sequence with the target lysine highlighted in red. The vertical axis indicates the residues that were sequentially exchanged at the position corresponding to the row. b Data from three independent experiments were averaged after normalizing the full activity to 100%. The activity is displayed in gray-scale as indicated. c Discrimination factors for the recognition of each amino acid at the corresponding position of the H3 substrate by NSD2 represented as a bar diagram.
