Fig. 5: IAAG on-grid purified CCT6-HR from cell lysates for high-resolution cryo-EM. | Communications Biology

Fig. 5: IAAG on-grid purified CCT6-HR from cell lysates for high-resolution cryo-EM.

From: An immobilized antibody-based affinity grid strategy for on-grid purification of target proteins enables high-resolution cryo-EM

Fig. 5: IAAG on-grid purified CCT6-HR from cell lysates for high-resolution cryo-EM.

a Cryo-EM images of the control GO grid treated with CCT6-HR cell lysates, indicating barely any CCT6-HR could be identified in the control grid. b Cryo-EM images of the IAAG on-grid purified CCT6-HR from cell lysates. Obviously, more CCT6-HR particles in distinct top/side views can be recognized. Here top view indicated by a red square, and the side view is in a yellow square. c The 2.8-Å-resolution cryo-EM map of CCT6-HR reconstructed from the IAAG data. d Local resolution estimation of the CCT6-HR complex. e High-resolution structure features of the CCT6-HR complex (PDB: 5GW5 subunit CCT6).

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