Table 1 Cryo-EM reconstruction statistics
| Â | Apoferritin (EMDB-38143) | CCT6-HR-ATP-AlFx (EMDB-38142) | TBCA-apoferritin-consensus (EMDB-38145) | TBCA-focused refined TBCA-apoferritin (EMDB-39651) | CCT6-HR (EMDB-38147) |
|---|---|---|---|---|---|
Data collection | |||||
 Microscope | Titan Krios | Glacios | |||
 Camera | K2 | K3 | Falcon 4i | ||
 Magnification | 29,000 | 81,000 | 130,000 | ||
 Voltage (kV) | 300 | 300 | 200 | ||
 Electron exposure (e−/Å2) | 41 | 56.8 | 50 | ||
 Defocus range (μm) | −1.0 ~ −2.0 | −0.6 ~ −2.5 | −0.6 ~ −2.5 | ||
 Pixel size (Å) | 0.81 | 0.86 | 0.89 | ||
Reconstruction | |||||
 Software | RELION 3.1 | RELION 3.1 & cryoSPARC 4.2.1 | |||
 Symmetry imposed | O | D8 | O | C1 | D8 |
 Initial particle images (no.) | 107,179 | 440,563 | 145,772 | 898,560 | 391,997 |
 Final particle images (no.) | 43,720 | 144,370 | 37,440 | 109,152 | 83,979 |
 Map resolution (Å) | 2.5 | 2.6 | 2.4 | 2.8 | 2.8 |
 Map resolution range (Å) | 2.4–3.0 | 2.5–3.0 | 2.2–4.0 | 2.5–5.0 | 2.5–3.5 |
 FSC threshold | 0.143 | ||||
