Fig. 2: The ability of S. coelicolor M145 and other Streptomycetes to degrade BHET.
From: Polyester degradation by soil bacteria: identification of conserved BHETase enzymes in Streptomyces
a Degradation of BHET by S. coelicolor after 18 days of growth on StrepMM Difco agar with and without Mannitol, BHET, and GlcNAc. b The degradation of BHET by S. coelicolor in liquid medium (n = 3). The control samples are displayed as control and only contain NMM with BHET [10 mM]. The addition of GlcNAc [25 mM] to the cultures is indicated with an asterisk. The area percentages were calculated using GraphPad. The areas are presented in percentage compounds present in the culture. BHET is indicated in turquoise and MHET is indicated in magenta. Some impurities are present as a peak around 9.5 min retention time, this compound is called unknown and is presented in purple. c Individual screen of all active strains on StrepMM Difco agar with and without mannitol, BHET, and GlcNAc after 10 days of growth. d Analysis of BHET degradation in agar plugs after 15 days of growth using LC-MS. The area percentage was calculated using GraphPad. *The agar plug of M145 was taken after 18 days. BHET is indicated in turquoise and MHET is indicated in magenta, TPA is represented in black. The statistical significance of the BHET degradation compared to the control is indicated with three asterisks. All error bars display the standard deviation, n = 3, and ***p < 0,0001. Individual data points are shown in Supplementary Fig. 4.
