Fig. 4: Expression of the LipA variants in S. coelicolor M145 ΔlipA in NMM and TSBS medium.
From: Polyester degradation by soil bacteria: identification of conserved BHETase enzymes in Streptomyces
a SDS-PAGE of concentrated samples on NMM medium, faint band around ~32 kDa in the overexpression strains, this band is not present in wild-type strain M145. b Western blot of NMM samples showing clear signal around ~32 kDa. c Analysis of BHET degradation in NMM of the wild-type strain, ΔlipA, S3, S5, and S7 using LC-MS (n = 3). Samples were taken at 24 h, 48 h and 72 h. The percentage of BHET is presented in turquoise, the percentage of MHET in magenta, and the percentage of TPA in black. The area percentage was calculated using GraphPad. d SDS-PAGE of concentrated samples on TSBS medium, a faint band around ~32 kDa was observed in the overexpression strains this band was not present in wild-type strain M145. e Western blot of TSBS samples showing a clear signal around 32 kDa. f Analysis of BHET degradation in TSBS of the wild-type strain, ΔlipA, S3, S5 and S7 using LC-MS. Samples were taken at 24 h, 48 h and 72 h. The percentage of BHET is presented in turquoise, the percentage of MHET in magenta, and the percentage of TPA in black. The error bars display the standard deviation. All original gel pictures are shown in Supplementary Fig. 8. The graphs containing all individual data points are shown in Supplementary Fig. 9.
