Fig. 6: Expression and purification of Lipase A enzymes in E. coli.
From: Polyester degradation by soil bacteria: identification of conserved BHETase enzymes in Streptomyces
a SDS-PAGE of the purified Lipase A variants, along with the PET40 (40), TfCut2, LCC, IsPETase (PET) and PET46 (46); 1: Ladder, multicolor broad range protein ladder; 2: S2LipA sample (30 kDa); 3: S92LipA (30 kDa); 4: ScLipA (30 kDa), 5: PET40 (28 kDa), 6: TfCut2 (28 kDa), 7: LCC (27 kDa), IsPETase (27 kDa), 8: PET46 (30 kDa). The Lipase variants show two bands one around 60 kDa and one around 35 kDa. b Western blot using a His-antibody was conducted to identify the purified enzymes of the Lipase A variants. 1: ladder, Spectra multicolor broad range protein marker; 2 till 8: same samples as for the SDS-PAGE c Zymogram of 750 ng of purified enzymes and LipA variants on 1% tributyrin. The Lipase A variants, PET40, TfCut2, LCC, and PETase show degradation of tributyrin (yellow spots). NativeMarker used as a marker and visualized by overlaying Native SDS-PAGE with a zymogram. All original gel pictures are shown in Supplementary Fig. 10.
