Fig. 7: Determination of optimal enzyme conditions and BHET degradation under optimum conditions.
From: Polyester degradation by soil bacteria: identification of conserved BHETase enzymes in Streptomyces
a–c Relative activity on para-nitrophenol dodecanoate at different pH for S2LipA (a), S92LipA (b) and ScLipA (c) (n = 3). The graphs are overlays of the graph containing the error bars and the same graph with the individual data points original graphs are displayed as Supplementary Fig. 13. d Enzymatic BHET degradation using colorimetric assay after 24 and 48 h. The activity of buffer control (black) as a negative control, S2LipA (magenta) S92LipA (turquoise), and ScLipA (dark purple), the positive controls are displayed as PET40 (lavender), TfCut2 (light blue) LCC (black), PET46 (dark blue), and IsPETase (purple). The activity was displayed as the concentration of BHET degraded in mM. The indicated significance displays the significant difference with the buffer (n = 3). The error bars display the standard deviation, ***p < 0,0001.
