Fig. 4: Cellular Wolbachia abundance in late-stage oocytes generally does not align with maternal transmission rates.

Cellular Wolbachia abundance in stage 10 oocytes (measured as fluorescence due to propidium iodide; CTCF), measured in a the whole oocyte, b the posterior region, and c the posterior cortex (N = 283 oocytes). Asterisks indicate that Wolbachia abundance differs between 25° and 20 °C for a given Wolbachia strain according to a Wilcoxon rank-sum test at P < 0.05. d Representative confocal images of Wolbachia strains that have decreased abundance at the posterior cortex in the cold (wMel), increased abundance at the cortex in the cold (wRi), and no change in the cold (wCha). Confocal micrographs are DNA-stained with PI (red) and actin-stained with phalloidin (green). The second column depicts a single channel image of the PI stain and the third column depicts an enlarged PI-stained image at the posterior cortex of each oocyte. Scale bars are set to 25 µm.