Fig. 5: RARA promotes the transcription of TXN and PPM1F by binding to their promotors. | Communications Biology

Fig. 5: RARA promotes the transcription of TXN and PPM1F by binding to their promotors.

From: Retinoic acid receptor alpha inhibits ferroptosis by promoting thioredoxin and protein phosphatase 1F in lung adenocarcinoma

Fig. 5: RARA promotes the transcription of TXN and PPM1F by binding to their promotors.

a Genome-wide data of TXN from the ENCODE database and our CHIP results showed the RARA-binding peak in the promotor region close to the TSS of TXN. b Predicted binding sequences of RARA in TXN showed by JASPAR. c Predicted BS of RARA in TXN and design of primers for ChIP-qPCR. d Genome-wide data of PPM1F from the ENCODE database and our CHIP results showed the RARA-binding peak in the promotor region close to the TSS of PPM1F. e Predicted binding sequences of RARA in PPM1F showed by JASPAR. f Predicted BS of RARA in PPM1F and design of primers for ChIP-qPCR. g ChIP-seq results demonstrated the enrichment of RARA to TXN and PPM1F in A549 and PC9 cells. (n = 3 biologically independent experiments, Student t-test). h Dual-luciferase assays showed the fluorescence intensity of RARA-NC and RARA-KD using luciferase plasmids containing WT or mutated type of the promoter region of TXN and PPM1F. (n = 3 biologically independent experiments, Student t-test) BS binding sites, ChIP chromatin immunoprecipitation, TSS transcription start site, WT wild type. ns, not significant; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.

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