Fig. 3: TIGIT⁺KLRG1⁺ CD57⁺ Tex population has increased single-cell gene expression heterogeneity and reciprocal expression of Inhibitory Receptors.

A Schematic of scRNA-seq sampling strategy; n = 12 donors, 1 sample per donor (6 R, 6 NR, sampled at Visit 30, 104 wk post-treatment). B UMAP dimensionality reduction of all cells clustered using the Leiden clustering method, with branching lines depicting the cell trajectory determined by Monocle. Phenotype assignment labels were based on Seurat reference mapping of CD8⁺ T cell phenotypes and expression of Tex markers (Supplementary Figs. 6c,  8)¹⁰. C Z-score adjusted mean expression in each cluster of known key markers of exhaustion and PD-1⁺ and CD57⁺ Tex populations, where Inhibitory Receptors (IRs, orange highlight) displayed a reciprocal expression pattern between PD-1⁺ and CD57⁺ Tex populations. D Log10 normalized single-cell gene expression of key exhaustion-related genes. More differentiated cells on the left of the UMAP dimensionality reduction display higher exhaustion-related gene expression.