Table 2 Primers used in this study

oT568

deletion of TLC1 (yT)

5’-GCA ATG GTG ACA TAT AGA TCT CAA GGT TCT CAA TTA AAA GAC CTT CTT TGT AGC TTT TAG TGT GAT TTT TCT GGT TTG AGC GGA TCC CCG GGT TAA TTA A-3’

oT1569

deletion of TLC1 (yT)

5’-GAC AAT TAC TAG GAT GTT CTT CTA TTT TTT TAT TTT TAT TTG TAT ATT GTA TAT TCT AAA AAG AAG AAG CCA TTT GGT GGG AAT TCG AGC TCG TTT AAA C-3’

oT210

insertion of P_TetO2 upstream of TLC1 (yT787)

5’- AAT ACG ATT AAG CAA ACG CAA CAG CCA TTG ACA TTT TCA TAG GGT ACC TAT CTT CCT CTC ATA GGC CAC TAG TGG ATC TG-3’

oT543

insertion of P_TetO2 upstream of TLC1 (yT787)

5’-AAA AAA CTT CCT CTT TAG CAA TGG TGA CAT ATA GAT CTC AAG GTT CTC AAT TAA AAG ACC CGG ATC CCC GGG TTA ATT AA -3’

oT1550

deletion of HOG1 (yT1473)

5’-GGT AAA TAC TAG ACT CGA AAA AAA GGA ACA AAG GGA AAA CAG GGA AAA CTA CAA CTA TCG TAT ATA ATA CGG ATC CCC GGG TTA ATT AA-3’

oT1551

deletion of HOG1 (yT1473)

5’-CCA TAA AAA AAA GAA ACA TCA AAA AGA AGT AAG AAT GAG TGG TTA GGG ACA TTA AAA AAA CAC GTG AAT TCG AGC TCG TTT AAA C-3’

oT1252

deletion of PBS2 (yT1714)

5’-ATT ATT ATA TTA AGC AGA TCG AGA CGT TAA TTT CTC AAA GCG GAT CCC CGG GTT AAT TAA-3’

oT1253

deletion of PBS2 (yT1714)

5’-TAT ATT CAC GTG CCT GTT TGC TTT TAT TTG GAT ATT AAC GGA ATT CGA GCT CGT TTA AAC-3’

oT1415

Mec1-21 point mutation (yT1743-yT1744)

5’-AAA CTA CAG GAT AAT ATC TTG TTT T-3’

oT1416

Mec1-21 point mutation (yT1743-yT1744)

5’-AAG ATA TTA TCC TGT AGT TTG GAT CA-3’

370

deletion of ATG8 (yT1701- yT1702)^a

5’-GAT AAG AGA ATC TAA TAA TTG TAA AGT TGA GAA AAT CAT AAT AAA-3’

371

deletion of ATG8 (yT1701- yT1702)^a

5’-CGA TTT TAG ATG TTA ACG CTT CAT TTC TTT TCA TAT AAA AGA CTA-3’

333

deletion of ATG32 (yT1705- yT1706)^a

5’-GTC CTA ATC ACA AAA GCA AAA GCG TAC GCT GCA GGT CGA C-3’

369

deletion of ATG32 (yT1705- yT1706)^a

5’-AAG TGA GTA GGA ACG TGT ATG TTT GTG TAT ATT GGA AAA AGG TTA-3’

oT182

TeloPCR Y’ Fwd

5’-CTG TAG GGC TAA AGA ACA GGG-3’

oT169

TeloPCR Rev

5’-GCG GAT CCG GGG GGG GGG G-3’