Fig. 1: Identification of Rickettsia used in this study.

a Bird’-eye view of the assembled genome of Rickettsia used in this study. The outer circle to the inner circle provides six types of information: contig length, gene density, gene annotation (arrow indicated direction color implies the KEGG of genes), DNA sequencing data coverage, GC skew value, and GC content. b The maximum likelihood phylogenomic tree of the Rickettsia used, along with 25 other publicly available Rickettsiales species. Anaplasma phagocytophilum and Ehrlichia ruminantium were two outgroup species used to root the tree. Four different groups of Rickettsia—spotted fever group (SFG), typhus group (TG), transitional group (TRG), and ancestral group (AG)—were indicated with different colors of backgrounds. c Giemsa staining of the Rickettsia used in this study in Vero-81 cells. d ANI between R. heilongjiangensis TIGMIC and other R. heilongjiangensis strains. e UpSetR plot showing orthogroups shared among different R. heilongjiangensis strains.