Fig. 2: Growth behavior of C. crescentus strains in phosphate-rich and phosphate-free media.

Shown are growth curves of wild-type (CB15N), ΔphoB (JK2) and ΔpstS (JK158) cells in phosphate-rich (+Pi) (A) complex (PYE) or (B) minimal (M2G) medium or in (C, D) phosphate-free (−Pi) minimal medium (M2G-P). The cultures were inoculated (1:20) with cells cultivated to the exponential or stationary growth phase in PYE medium, as indicated in the graphs. Lines represent the average of three independent experiments. Shades indicate the standard deviation.