Fig. 7: Effect of protective OIT on fucosylation and fucosyltransferase expression in the gut tissue during arthritis. | Communications Biology

Fig. 7: Effect of protective OIT on fucosylation and fucosyltransferase expression in the gut tissue during arthritis.

From: Undenatured type II collagen protects against collagen-induced arthritis by restoring gut-joint homeostasis and immunity

Fig. 7

a, b Ileum and colon sections from naïve, CIA and asymptomatic OIT mice were stained with UEA (a) and AAL (b) biotinylated lectins and fluorescence streptavidin (yellow) to detect terminal and core fucosylation respectively. DAPI (Blue) was used as counterstaining. Scale bars: 500 μm. Images show one representative example of each group. Graphs show the mean pixel intensity for lectin staining in individual mice; each dot shows the mean value from 10 different analysed areas, quantified using Image J software. c, d Relative expression of fucosyltransferases mRNA was evaluated by RT-PCR in the ileum (c) and colon (d) samples, including FUT1, FUT2, FUT4, FUT7, FUT8 and FUT9. Expression is shown as relative to actin expression. Statistical significance was evaluated by one-way ANOVA where *p < 0.05. Each dot represents values of individual mice where data are collected from two independent experiments. Error bars represent standard error (SEM).

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