Table 3 Primers used for creating engineered strains

5′up_ura3

CAACTCCCGCCAGCGAATTGGCTACTAATGGCGGT

For upstream of URA3

3′up_dw

CAGTACGCCAGCGTCAATACACTCCCGTCAATTAGTTGCACCTTATACAGGAAAC

5′up_dw

TCCCGTCAATTAGTTGCACCTTATACAGGAAACTTAATAGAACAAATCACATATTTAATC

For downstream of URA3

3′dw_ura

TGGCGACTTGATTTATTATAACAACGACAATAGG

ura3ko_F

CTTATCACGACTATAAAGCCA

Nested PCR to create the URA3 knockout DNA fragment

ura3ko_R

GTTCTACTTGATCAGAGGAGT

5′ace2flk_ura3

ATGTCAGATCGGAACCGTACGCTGCAGGTCGACGGATCCCCCTGGCACGACAGGTTTCCC

To create URA3 gene fragment with ACE2 flanking regions. In order to construct Y1140-ACE2::URA3

3′ura3_ace2flk

TCCGTTGATATTAGCAGTTTATCGCCTCGACATCATCTGCCCAGATGCGAAGTTAAGTGC