Fig. 2: Representative morpho-chemical information collected through RS and TPM in human colon cancer-derived cells.

Cell cycles were not synchronized through the work, so as to make results agnostic to sampled cell cycle phases (Supplementary Fig. 12 and Supplementary Fig. 13). a, e, i RS microscopy images of an HT29, RKO, and T84 cell, obtained integrating the hypercubes along the Ω dimension in the indicated spectral region. b, f, j On the left, cell average spectrum (in black) ± standard deviation (in blue), and single-point spectra of nuclei (in blue, Ω = 780–790 cm⁻¹) and lipids (in red, Ω = 1440–1450 cm⁻¹), compared to the cell mean (in black) and substrate mean (in grey). c, g, k Set of quantitative information extracted from the TPM RI maps of the cell. d, h, l Sections of the cell tomogram, along the axial, sagittal, and coronal planes. Scale bars: 10 µm in both RS and TPM images.