Fig. 3: PCA-MLR analysis results on the dataset of cell-averaged Raman spectra measured in living human colon cancer cell lines.

a MLR fitting equation and estimated model coefficients (βi) with their statistical significance (p-values) by via a two-tailed Student t-test. In grey, low-significance (p-value < 0.05) β coefficients. In red, high-significance (p-value < 0.01) β coefficients. b Partial leverage plots of significant PCs in the MLR model. c PCA loadings with indication of relevant Raman peaks. Red PCs are associated to highly significant β coefficients, the grey PC features lower significance. Sample size = 5 independent cells/phenotype.