Fig. 4: MiR22-sEVs promoted RGC viability and suppressed RGC apoptosis in NMDA-induced RGC injury in vitro. | Communications Biology

Fig. 4: MiR22-sEVs promoted RGC viability and suppressed RGC apoptosis in NMDA-induced RGC injury in vitro.

From: Small extracellular vesicles derived from microRNA-22-3p-overexpressing mesenchymal stem cells protect retinal ganglion cells by regulating MAPK pathway

Fig. 4

a CCK-8 analysis of cell viability in each group. Data are presented as means ± SD from 5 independent experiments. b Annexin V/PI flow cytometry analysis of RGC apoptosis in each group. c Western blotting analysis of Bax protein expression in each group. d Western blotting analysis of caspase-3 protein expression in each group. Data are presented as means ± SD from 3 independent experiments. e Western blotting analysis of cleaved PARP protein expression in NMDA-induced RGC-5 injury model with or without Z-VAD-FMK pretreatment. *P < 0.05, **P < 0.01, ***P < 0.005, ****P < 0.001.

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