Fig. 6: MiR22 targets to 3’UTR of Map3k12 and effects on MAPK pathway. | Communications Biology

Fig. 6: MiR22 targets to 3’UTR of Map3k12 and effects on MAPK pathway.

From: Small extracellular vesicles derived from microRNA-22-3p-overexpressing mesenchymal stem cells protect retinal ganglion cells by regulating MAPK pathway

Fig. 6

a The seed sequence of miR22 is complementary to Map3k12 3’UTR and is highly conserved among different species. b Binding site of Map3k12 3’UTR (WT-3’UTR) with miR22 and designed Map3k12 3’UTR mutant sequence (Mutant-3’UTR). c miR22 reduced the luciferase activity of the vector with Map3k12 3’UTR, but the mutant version abrogated the repressive ability. Data are presented as means ± SD from 5 independent experiments. d Western blotting presentation of MAP3K12, ERK, p38, and caspase-3 in RGC-5 cell line post transfected with miR22 mimics or miR22 inhibitors. ***P < 0.005.

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