Fig. 1: Regulation of G proteins trafficking from the plasma membrane to the endosomes by Gβγ and βarr.
From: Role of the V2R–βarrestin–Gβγ complex in promoting G protein translocation to endosomes
a AVP-induced (100 nM) Gαs dissociation from the plasma membrane after 20 min stimulation monitored by ebBRET between Gαs67-RlucII and rGFP-CAAX in parental HEK293SL cells and βarr1 and βarr2 KO (βarr1/2 KO) cells. Overexpression of βARKct-CAAX and Gβ1γ2 modulates Gαs dissociation from the plasma membrane. b AVP-induced (100 nM) Gαs dissociation from the plasma membrane after 20 min stimulation monitored by ebBRET between Gαs67-RlucII and rGFP-CAAX in parental HEK293SL cells. Overexpression of βARKct-FYVE and Gβ1γ2 modulates Gαs dissociation from the plasma membrane. c AVP-induced (100 nM) Gαs trafficking to the endosomes after 20 min stimulation monitored by ebBRET between Gαs67-RlucII and rGFP-FYVE in parental HEK293SL cells and βarr1/2 KO cells. Overexpression of βARKct-CAAX and Gβ1γ2 modulates Gαs translocation to early endosomes. d AVP-induced (100 nM) Gαs trafficking to the endosomes after 20 min stimulation monitored by ebBRET between Gαs67-RlucII and rGFP-FYVE in parental HEK293SL cells. Overexpression of βARKct-FYVE and Gβ1γ2 modulates Gαs translocation to early endosomes. e AVP-induced (100 nM) Gβγ internalization after 20 min stimulation monitored by ebBRET between Gγ2-RlucII and rGFP-CAAX in parental HEK293SL cells and βarr1/2 KO cells with or without βarr1 and βarr2 supplementation (βarr1/2 indicating that both βarrs were co-transfected). f AVP-induced (100 nM) Gβγ endosomal trafficking after 20 min stimulation monitored by ebBRET between Gγ2-RlucII and rGFP-FYVE in parental HEK293SL cells and βarr1/2 KO cells with or without βarr1 and βarr2 supplementation. Data are represented as the mean ± SEM (n = 5) and statistical significance of the differences was assessed using a two-way ANOVA followed by Holm-Šídák’s multiple comparison test (ns nonsignificant; *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001; ****P ≤ 0.0001).
