Fig. 2: CXCR4-mediated Gβγ trafficking is βarr-dependent.
From: Role of the V2R–βarrestin–Gβγ complex in promoting G protein translocation to endosomes
a V2R internalization is monitored by ebBRET using V2R-RlucII and rGFP-CAAX in parental HEK293SL cells and βarr1 and βarr2 KO (βarr1/2 KO) cells with or without βarr1 and βarr2 supplementation after 20 min 100 nM AVP stimulation. b CXCR4 internalization is monitored by ebBRET using CXCR4-RlucII and rGFP-CAAX in parental HEK293SL cells and βarr1/2 KO cells with or without βarr1 and βarr2 supplementation after 20 min 100 nM CXCL12 stimulation. c CXCL12-induced (100 nM) Gβγ internalization after 20 min stimulation monitored by ebBRET between Gγ2-RlucII and rGFP-CAAX in parental HEK293SL cells and βarr1/2 KO cells with or without βarr1 and βarr2 supplementation. Data are represented as the mean ± SEM (n = 4–5) and statistical significance of the differences was assessed using a two-way ANOVA followed by Holm-Šídák’s multiple comparison test (ns nonsignificant; **P ≤ 0.01; ***P ≤ 0.001; ****P ≤ 0.0001).
