Fig. 3: Contour shape analysis on deformed membranes.
a Snapshots showing the definition of radial membrane position R(θ, t) and deformation amplitude u(θ, t). b Time evolution of the deformation amplitude in mixed architecture cortex. Time is color-coded. The right snapshots show the mid-plane image of the membrane at 0 min and 2 min. c Time evolution of the deformation amplitude in Arp2/3-nucleated cortex. d Time evolution of the deformation amplitude in mDia1-nucleated cortex. e Boxplot showing maximum radial deformation (n = 24 liposomes and N = 2 independent experiments in Arp2/3; n = 31 and N = 2 in mDia1; n = 31 and N = 3 in Mixed). f Power spectrum of membrane deformation with different F-actin cortex architecture. The power spectrum is averaged over 20 frames for each liposome, which is averaged for all liposomes. Curves are mean ± SEM. Dotted lines are the eye guide for q−4 and q−2 (n = 14 and N = 2 in Arp2/3; n = 13 and N = 2 in mDia1; n = 15 and N = 2 in Mixed). g Boxplot showing scaling exponent α. Scaling exponent is extracted by fitting the power spectrum by a function q−a for all q (n = 20 and N = 2 in Arp2/3; n = 26 and N = 2 in mDia1; n = 30 and N = 3 in Mixed). h Autocorrelation of the deformation amplitude. θc is the deformation size calculated at the smallest θ at which autocorrelation becomes 0. The autocorrelation at the time frame t' was shown at which θc takes the smallest value. Curves are mean ± SD (n = 14 and N = 2 in Arp2/3; n = 13 and N = 2 in mDia1; n = 15 and N = 2 in Mixed). i Boxplot showing the deformation size (n = 33 and N = 3 in Arp2/3; n = 32 and N = 3 in mDia1; n = 49 and N = 3 in Mixed). *, **, and *** represent p < 0.05, p < 0.01 and p < 0.001, respectively. n.s. not significant. Scale bars, 10 μm.
