Fig. 2: OLFM4 is required for IL-22⁺ILC3s in pathogen resistance.

A The experimental scheme of C. rodentium infection mouse model. WT and OLFM4^−/− mice were orally inoculated with 1 × 10⁸ CFU of C. rodentium. Samples were harvested on day 7 post-infection. B Percentage of the initial weight during colitis (n = 9). C Comparison of survival rates between indicated mice at 7dpi. D Spleen weight of indicated mice after infection (n = 7). E Histological score of the colon at 7 dpi (n = 7). Log10 CFU of C. rodentium in (F) liver and (G) spleen (n = 7). H Statistical results of proportion and absolute numbers of CD4⁺RORγt⁺ cells (n = 5). I Representative FACS plots and statistical results of the proportion and absolute numbers of ILC3 in LPMCs from indicated mice (n = 7). J Statistical results of proportion of the of NKp46 + ILC3 (left) and CCR6 + ILC3 (right) in LPMCs (n = 6). K Representative FACS plots of IL-22 expressing ILC3 in LPMCs mice during colitis. L Statistical results of proportion and absolute numbers of IL-22-expressing ILC3 (n = 7). M mRNA levels of Reg3b, Reg3g, Muc1, and Cldn2 in colon tissues (n = 4). Data are presented as mean ± SEM, and statistical significance was determined using a two-tailed unpaired Student’s t-test (B, D-J, L, M). Kaplan–Meier analysis and survival curves were performed for survival analysis (C). ns, not significant; *P < 0.05; **P < 0.01; ***P < 0.001. The experiments were performed three times (at least three replicates).