Fig. 2: Molecular mechanism of nanoalgosome internalization in human cell lines and in vivo. | Communications Biology

Fig. 2: Molecular mechanism of nanoalgosome internalization in human cell lines and in vivo.

From: Extracellular vesicles from the microalga Tetraselmis chuii are biocompatible and exhibit unique bone tropism along with antioxidant and anti-inflammatory properties

Fig. 2

a Effect of metabolic inhibitors of endocytosis on nanoalgosome uptake in 1–7 HB2 cells treated with EIPA, nystatin and dynasore. Results are presented as arbitrary unit of nanoalgosome fluorescence intensity inside cells after 2 h and 3 h of incubation. Each value represents the mean ± SD from three independent experiments (n = 3 biologically independent experiments). One-way ANOVA statistical test was used to assess the statistical significance of the differences: 37 °C (2 h) (3 h) vs 4 °C (2 h) (3 h), EIPA 10 µM (2 h) (3 h), nystatin 50 µM (2 h) (3 h) and dynasore 60 µM (2 h) (3 h), ****p < 0.0001, *p < 0.01, ns=not significant (p > 0.5). b Representative confocal images of wild-type and chc-1 KO heterozygote C. elegans animals after 24 h of treatment with Di-8-ANEPPS labeled nanoalgosomes (red). Fluorescent signal is observed only in the intestinal cells of wild-type animals, while in chc-1 KO heterozygote animals the fluorescent signal is strongly reduced. Animal body is outlined with white lines. Scale bar is 50 µm.

Back to article page