Fig. 3: Both cspC-82 and cspC-1718 mutants show a differential response to bile salt germinants. | Communications Biology

Fig. 3: Both cspC-82 and cspC-1718 mutants show a differential response to bile salt germinants.

From: Clostridium septicum manifests a bile salt germinant response mediated by Clostridioides difficile csp gene orthologs

Fig. 3: Both cspC-82 and cspC-1718 mutants show a differential response to bile salt germinants.

Purified spores of wild-type (WT), cspC mutants (cspC-82::ermB and cspC-1718::ermB) and complemented cspC mutants (cspC-82::ermB pcspC-82 and cspC-1718::ermB pcspC-1718) were incubated with 10 mM bile salts in the presence of oxyrase. a Heat map depicting %OD drop values at the end of the assay. Colors indicate extent of germination (red: germination, yellow: no germination). The cspC-82 mutant was able to germinate only in the presence of GCA while the cspC-1718 mutant was able to germinate in the presence of CDCA and DCA. Complementation of the mutant with their respective gene rescued the mutant phenotype. Data represent the average of n = 2 biologically independent experiments. b Representative phase contrast images of WT, cspC mutants and their respective complemented cspC mutants after 900 minutes of incubation with various bile-salts. Scale bar, 2 μm.

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