Fig. 5: Pharmacological regulation of DR REV-ERVα altered the circadian depression-like phenotypes. | Communications Biology

Fig. 5: Pharmacological regulation of DR REV-ERVα altered the circadian depression-like phenotypes.

From: Role of the circadian nuclear receptor REV-ERBα in dorsal raphe serotonin synthesis in mood regulation

Fig. 5

a Experimental scheme for the behavioral tests for local microinjection of SR8278 (Rev-erbα antagonist), GSK4112 (Rev-erbα agonist), or vehicle (DMSO) into DR. SR8278, GSK4112, or vehicle was administered 3-h prior to the depression-like behavioral tests. b Quantification of 5-HT after local microinjection of either SR8278, GSK4112, or vehicle in DR at ZT04. Each animal was administered the selected drugs for three consecutive days before sampling. Data are presented as mean ± SEM, and significance was assessed by one-way ANOVA followed by Dunnett’s post-hoc test; *P < 0.05, **P < 0.01. Depression-like phenotypes were measured after SR8278, GSK4112, or vehicle microinjection into the DR. Immobility was reduced in FST (c) and TST (d) after SR8278 microinjection at dusk, whereas GSK4112 increased the immobility at dawn (n = 8 for controls; n = 6 for SR8278; n = 9 for GSK4112). Data are presented as mean ± SEM. Significance was assessed by two-way ANOVA followed by Tukey’s post hoc test; *P < 0.05, **P < 0.01, ****P < 0.0001, and ns = not significant.

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