Fig. 2: Effect of CBD on cell proliferation and osteogenic differentiation of DPSC in vitro. | Communications Biology

Fig. 2: Effect of CBD on cell proliferation and osteogenic differentiation of DPSC in vitro.

From: Dental pulp stem cells-derived cannabidiol-treated organoid-like microspheroids show robust osteogenic potential via upregulation of WNT6

Fig. 2

A Effect of 0.1, 0.5, 2.5, and 12.5 µM of CBD on the proliferation of DPSC (n = 6). ALP staining (B) and alizarin red staining (D) of DPSC cultured with CBD (0, 0.1, 0.5, and 2.5 µM). C ALP activity of DPSC cultured with CBD (n = 4). E Quantification of mineralized matrix in DPSC cultured with CBD (n = 4). The data are presented as mean ± SD. Significant difference compared with the respective control group, *P < 0.05, **P < 0.01, and ***P < 0.001. OM osteogenic medium.

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