Fig. 3: Microspheroids development in microwell method. | Communications Biology

Fig. 3: Microspheroids development in microwell method.

From: Dental pulp stem cells-derived cannabidiol-treated organoid-like microspheroids show robust osteogenic potential via upregulation of WNT6

Fig. 3

A PDMS mold with uniform microwells. B The schematic presentation of agarose microwells on PDMS and the microspheroids formation in microwells. C Representative bright-field images of DPSC to microspheroids in a time-dependent manner. Representative confocal z-projection images of cell cytoskeleton in microspheroids stained by Phalloidin actin (D), and Live/Dead cells in microspheroids stained by Calcein AM/PI. Red arrows were pointed to the dead cells (E). F The diameter of microspheroids over time. Scale bar 50 µm. The data are presented as mean ± SD (n = 3). OM osteogenic medium.

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