Fig. 2: Bulked segregant analysis of candidate HGT genes related to the high-temperature tolerance of P. haitanensis. | Communications Biology

Fig. 2: Bulked segregant analysis of candidate HGT genes related to the high-temperature tolerance of P. haitanensis.

From: Horizontal gene transfer and symbiotic microorganisms regulate the adaptive evolution of intertidal algae, Porphyra sense lato

Fig. 2

a, b Morphological characteristics of the heat-resistant strain (a) and the heat-sensitive strain (b) observed before (21 °C) and after the high-temperature treatment (7 days at 30 °C). c–e Physiological and biochemical indices of the HR-Pool (heat-resistant mixed pool) and HS-Pool (heat-sensitive mixed pool) following the high-temperature treatment (7 days at 30 °C). Measurements include c Fv/Fm, d daily increase in fresh weight, and e daily increase in length. f Bulked segregant analysis [Δ(SNP-index)] on the candidate intervals related to P. haitanensis thallus thermostability. The x-axis represents the physical positions of the P. haitanensis chromosomes. Red and blue lines indicate statistical significance at P < 0.01 and P < 0.05, respectively. Abbreviations in the box: sirohydrochlorin ferrochelatase (sirB), RNA polymerase primary sigma factor (rpoD), long-chain-fatty-acid-CoA ligase ACSBG (ACSBG), kinesin light chain (KLC).

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