Fig. 6: SIRT2 is a bona fide target of rhein.
From: Rhein targets macrophage SIRT2 to promote adipose tissue thermogenesis in obesity in mice
a, b BMDMs were primed with LPS and pretreated with SIRT2 inhibitor AGK2 for 2 h, and then treated with rhein or DMSO for 0.5 h and finally stimulated with ATP. a IL-1β levels detected by ELISA in the supernatant of BMDMs. b Immunoblots of Caspase1 and acetylated α-Tubulin in cell lysate of BMDMs. c The ratio of NAD+ to NADH in BMDMs and J774A.1 macrophages primed with LPS and then treated with rhein or DMSO for 0.5 h and finally stimulated with ATP. d SPR assays on recombinant mouse SIRT2 incubated with rhein at the indicated concentrations. e MST assays on cell lysate of HEK-293T cells transfected with SIRT2-GFP incubated with rhein at the indicated concentrations. f Merge of the catalytic domain and highly flexible region of SIRT2 captured molecular dynamics simulations. g Keyframe of rhein-SIRT2 combination captured in molecular dynamics simulations. The dashed lines represent hydrogen bonds. Data are presented as the mean ± SD. Significance is indicated by *p < 0.05 and ***p < 0.001 compared to the corresponding group. n.s. indicates no significance.
