Fig. 3: Expression level of IL-33 in the mouse uterus.

a Schematic diagram of experiments carried out in mice. The mice were randomly assigned to the following groups (30 in each group): the control group; the sham operation group (sham), in which the mice underwent laparotomy without any treatment; the IUA model group (IUA), in which mice underwent induction of previously described mechanical damage; the rmIL-33-treated group (IUA + IL-33), in which mice experienced intrauterine injection of rmIL-33 (4 µg) on day 0 on both sides of the uterus during the mechanical scratching process; the αIL-33-treated group (IUA + αIL-33), in which the mice received an intrauterine injection of αIL-33 (10 µg) on both sides of the uterus on day 0 during the mechanical scratching process. The mice from each group were killed on day 7. b–d qPCR and Western blotting were employed to analyze the mRNA and protein levels of IL-33 in the endometrium. The full-length blots/gels are presented in Supplementary Fig. 2 (e–i). The protein levels of IL-33 and fibrogenic cytokines in the uterus were determined via ELISAs. The data are from one experiment with three independent experiments with three mice per group (n = 3). The values are the means ± SDs. *p < 0.05, **p < 0.01, ***p < 0.001, ns denotes p > 0.05 (unpaired Student’s t test).