Fig. 5: Increase in the expression of endometrial stem cell markers under αIL-33 treatment.

a Schematic diagram of experiments carried out in mice. The mice were randomly assigned to the following groups (30 in each group): the control group; the sham operation group (sham), in which the mice underwent laparotomy without any treatment; the IUA model group (IUA), in which the mice underwent induction of previously described mechanical damage; the rmIL-33-treated group (IUA + IL-33), in which the mice experienced intrauterine injection of IL-33 (4 µg) on both sides of the uterus during the mechanical scratching process; and the αIL-33-treated group (IUA + αIL-33), in which the mice experienced intrauterine injection of αIL-33 (10 µg) on both sides of the uterus during the mechanical scratching process. The mice from each group were killed on day 7. b Western blotting was conducted to determine the protein levels of the stem cell markers in each group. The full-length blots/gels are presented, and statistical analysis of the quantitative results is presented in Supplementary Fig. 2. c qRT‒PCR was used to measure the mRNA expression levels of SOX9, SSEA1, VEGF, and FoxA2 in the endometrial tissues of each group. The data are from one experiment with three independent experiments with three mice per group (n = 3). The values are the means ± SDs. *p < 0.05, **p < 0.01, ***p < 0.001, ns denotes p > 0.05 (unpaired Student’s t test).