Fig. 8: IL-33 activates the formation of IUAs via the MAPK signaling pathway. | Communications Biology

Fig. 8: IL-33 activates the formation of IUAs via the MAPK signaling pathway.

From: Interleukin-33 promotes intrauterine adhesion formation in mice through the mitogen-activated protein kinase signaling pathway

Fig. 8: IL-33 activates the formation of IUAs via the MAPK signaling pathway.The alternative text for this image may have been generated using AI.

a Schematic diagram of mouse organoid culture and experiments carried out in mice. b Representative images of endometrial organoids treated with ST2 inhibitors at different time points were captured under a light microscope. The results of the gradient concentration experiment for ST2 are presented in Supplementary Fig. 4. c qRT‒PCR analysis of the mRNA expression levels of IL6, IL-1β, TGF-β, and α-SMA in endometrial organoids. d The protein expression levels of JNK, ERK, and p38 were measured via Western blotting after endometrial organoids were treated with the JNK inhibitor SP600125, the ERK/MEK1 inhibitor PD98059, or the p38 inhibitor SB203580 before rmIL-33 was added at 100 ng/ml. Full-length blots/gels are presented, and the results of the statistical analysis of the quantitative results are presented in Supplementary Fig. 5. The data are from one experiment with three independent experiments with three mice per group (n = 3). The values are the means ± SDs. *p < 0.05, **p < 0.01, ***p < 0.001, ns denotes p > 0.05 (unpaired Student’s t test).

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