Fig. 3: CDA regulates mitochondrial biogenesis in pancreatic cancer cells through mechanisms independent of its known catalytic activity. | Communications Biology

Fig. 3: CDA regulates mitochondrial biogenesis in pancreatic cancer cells through mechanisms independent of its known catalytic activity.

From: Cytidine deaminase-dependent mitochondrial biogenesis as a potential vulnerability in pancreatic cancer cells

Fig. 3: CDA regulates mitochondrial biogenesis in pancreatic cancer cells through mechanisms independent of its known catalytic activity.

A Metabolomic analysis of Mia PaCa-2 cells expressing WT CDA or E67Q CDA mutant. Cells expressing luciferase were used as control. Results are mean ± s.e.m. of three experiments. ATP adenosine triphosphate, UMP uridine triphosphate. B Succinate Dehydrogenase Complex Flavoprotein Subunit B (SDHB) activity in Mia PaCa-2 cells expressing WT CDA or E67Q CDA mutant, with cells expressing luciferase used as control. Results are mean ± s.e.m. of three experiments performed in triplicate. C Quantification of ATP production from OXPHOS in control Mia PaCa-2 cells and cells expressing WT CDA or E67Q CDA. Results are mean ± min to max of six experiments performed in triplicate. D Mitochondrial membrane potential analysis in control  Mia PaCa-2 cells and cells expressing WT CDA or E67Q CDA. Results are mean ± min to max of five experiments performed in triplicate. E Quantification of mitochondrial mass using mitotracker red (MTR) in control Mia PACA-2 cells and cells expressing WT CDA or E67Q CDA. Results are mean ± min to max of eight independent experiments performed in duplicate. F Mitochondrial DNA (mtDNA) content analysis by qPCR, in control  Mia PaCa-2 cells and cells expressing WT CDA or E67Q CDA. Results are mean ± min to max of six experiments performed in triplicate. G Western blot analysis of Mitochondrial transcription factor A (TFAM), Succinate Dehydrogenase Complex Flavoprotein Subunit B (SDHB), cytochrome c oxidase subunit 2 (Cox II), NADH:Ubiquinone Oxidoreductase Subunit B8 (NDUFB8), Isocitrate Dehydrogenase (NAD(+)) 3 Catalytic Subunit Alpha (IDH3A) and Succinate Dehydrogenase Complex Flavoprotein Subunit A (SDHA) expression in control Mia PaCa-2 cells and cells expressing WT CDA or E67Q CDA. Heat-shock protein 90 (HSP-90) expression was used as a control. Fold change in protein expression is indicated. Representative of three experiments. *p < 0.05, **p < 0.01, ***p < 0.005, ****p < 0.001.

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