Fig. 3: Val potentiates the membrane permeabilizing activity of colistin to cause membrane disruption.
From: Valnemulin restores colistin sensitivity against multidrug-resistant gram-negative pathogens
A SEM imaging of E. coli P47 treated with Val (2 μg/ml), colistin (2 and 64 μg/ml), and the combination of both (Val 2 μg/ml + colistin 2 μg/ml). B Determination of the bacterial membrane permeability of E. coli P47, K. pneumoniae P30, and A. pittii CS17 treated with Val (8 μg/ml), colistin (0, 1, 2 μg/ml), and their combination using SYTOX Green staining. Val valnemulin HCl, CT colistin. Experiments were performed in biological triplicate and the data were presented as means ± SD. The statistical significance of colistin plus Val versus colistin was analyzed using an unpaired t-test (****p < 0.0001). Statistical significance of colistin plus Val versus Val was determined by one-way ANOVA, and shown as ####p < 0.0001.
