Fig. 1: HIV-1 infection monotonically increases with the number of receptive anal intercourse partners.

The X-axis categorizes participants (N = 241) into exposure groups, ranging from Group 1 (G1) through Group 4 (G4). The Y-axis represents the percentage distribution of the participants with subsequent HIV-1 infection (Case, depicted in red) and negative controls (Ctrl, depicted in blue). Combined, these percentages total 100%. As participants move from G1 to G4, indicative of an increase in the number of partners, there is a marked rise in the percentage of HIV infection. This increase is statistically significant supported by the likelihood ratio test (LRT) statistics of 0.0021 and a p-value (one-sided) of less than 0.001, as determined by the constrained linear mixed effects (CLME) test, suggesting a monotonic increasing trend.