Fig. 3: Concordance between single-cell whole transcriptome and BCR libraries.

UMAP of isotypes of BCR heavy chain sequences recovered from single-cell libraries prepared by Seq-Well (a) and 10x Genomics 3‘ GEX (b), respectively. c, d Distribution of recovered BCR isotypes among B cells binned by the most common Ig constant region transcript present in WT. c is for Seq-Well-prepared samples, and (d) for 10x Genomics 3‘ GEX. All cells shown have at minimum 3 counts of transcript detected in the WTA product. sotypes of BCR heavy chain sequences recovered from single-cell libraries, grouped by phenotypes assigned in single-cell gene expression data from Seq-Well (e) and 10x Genomics 3‘ GEX (f), respectively. All genes shown have at least two transcripts recovered in both WTA and BCR libraries. g, h Heat map comparing most common heavy chain V gene segments in WT libraries and V-genes of recovered BCR sequences. Heavy chain somatic mutation frequency overlaid onto UMAP of cells prepared by Seq-Well (i) and 10x Genomics 3‘ GEX (j), respectively. k, l Somatic mutation frequency of heavy chain BCR sequences grouped by B cell phenotypes. m, n Somatic mutation frequency of light chain BCR sequences grouped by B cell phenotypes. k, m are for Seq-Well-prepared samples, and (n) for 10x Genomics 3‘ GEX. o, p Somatic mutation frequency of BCR sequences grouped by B cell isotypes. P values are calculated using a two-sided Wilcoxon rank-sum test and are adjusted using Bonferroni correction. ns p > 0.05, *p < = 0.05, **p <= 0.01, ***p <= 0.001, ****p <= 0.0001.