Fig. 4: Accuracy and sensitivity of the approach. | Communications Biology

Fig. 4: Accuracy and sensitivity of the approach.

From: Full-length single-cell BCR sequencing paired with RNA sequencing reveals convergent responses to pneumococcal vaccination

Fig. 4

a UMAP of transcriptional phenotypes present in B cell-enriched PBMC with spike-in of U6-transfected HEK cell line. b Dot plot showing scaled expression and the fraction of cells expressing B cell marker genes. c UMAP of BCR recovery. d Fraction of BCR recovered from each transcriptional phenotype. e Relationship of heavy and light chain sequences recovered from HEK cells to U6 BCR. f Cell phenotypes with recovery of U6 BCR. Correlation between IGHV (g) and IGKV/IGLV (h) frequency in recovered BCR from single cells and BCR from 5‘-RACE bulk sequencing. Pearson’s correlation coefficient and the associated p value are shown.

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